FHL1 Antikörper (C-Term)

Produktnummer ABIN2775759

Dieser Anti-FHL1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von FHL1 in WB und IHC. Geeignet für Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Schaf, Pferd und Kaninchen. Dieses Primary Antibody wurde in 12+ Publikationen zitiert.

Kurzübersicht für FHL1 Antikörper (C-Term) (ABIN2775759)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Schaf, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-FHL1 Antikörper

Bindungsspezifität: C-Term

Sequenz: YYCVDCYKNF VAKKCAGCKN PITGFGKGSS VVAYEGQSWH DYCFHCKKCS

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against FHL1. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FHL1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 280 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-FHL1 Antikörper (ABIN2775759)

Le Thanh, Meinke, Korfali, Srsen, Robson, Wehnert, Schoser, Sewry, Schirmer: "Immunohistochemistry on a panel of Emery-Dreifuss muscular dystrophy samples reveals nuclear envelope proteins as inconsistent markers for pathology." in: Neuromuscular disorders : NMD, Vol. 27, Issue 4, pp. 338-351, (2017) (PubMed).

Xue, Schoser, Rao, Quadrelli, Vaglio, Rupp, Beichler, Nelson, Schapacher-Tilp, Windpassinger, Wilcox: "Exome Sequencing Identified a Splice Site Mutation in FHL1 that Causes Uruguay Syndrome, an X-Linked Disorder With Skeletal Muscle Hypertrophy and Premature Cardiac Death." in: Circulation. Cardiovascular genetics, Vol. 9, Issue 2, pp. 130-5, (2016) (PubMed).

Ma, Mao, Shen, Zheng, Li, Liu, Ni: "Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 353-9, (2014) (PubMed).

Fujii, Hayashi, Kawamura, Higuchi, Tsugawa, Ohyagi, Hayashi, Nishino, Kira: "A case of adult-onset reducing body myopathy presenting a novel clinical feature, asymmetrical involvement of the sternocleidomastoid and trapezius muscles." in: Journal of the neurological sciences, Vol. 343, Issue 1-2, pp. 206-10, (2014) (PubMed).

Koike, Kasamatsu, Iyoda, Saito, Kouzu, Koike, Sakamoto, Ogawara, Tanzawa, Uzawa: "High prevalence of epigenetic inactivation of the human four and a half LIM domains 1 gene in human oral cancer." in: International journal of oncology, Vol. 42, Issue 1, pp. 141-50, (2012) (PubMed).

Sharma, Shathasivam, Ignatchenko, Kislinger, Gramolini: "Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis." in: Molecular bioSystems, Vol. 7, Issue 4, pp. 1185-96, (2011) (PubMed).

Poparic, Schreibmayer, Schoser, Desoye, Gorischek, Miedl, Hochmeister, Binder, Quasthoff, Wagner, Windpassinger, Malle: "Four and a half LIM protein 1C (FHL1C): a binding partner for voltage-gated potassium channel K(v1.5)." in: PLoS ONE, Vol. 6, Issue 10, pp. e26524, (2011) (PubMed).

Schoser, Goebel, Janisch, Quasthoff, Rother, Bergmann, Müller-Felber, Windpassinger: "Consequences of mutations within the C terminus of the FHL1 gene." in: Neurology, Vol. 73, Issue 7, pp. 543-51, (2009) (PubMed).

Gueneau, Bertrand, Jais, Salih, Stojkovic, Wehnert, Hoeltzenbein, Spuler, Saitoh, Verschueren, Tranchant, Beuvin, Lacene, Romero, Heath, Zelenika, Voit, Eymard, Ben Yaou, Bonne: "Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy." in: American journal of human genetics, Vol. 85, Issue 3, pp. 338-53, (2009) (PubMed).

Windpassinger, Schoser, Straub, Hochmeister, Noor, Lohberger, Farra, Petek, Schwarzbraun, Ofner, Löscher, Wagner, Lochmüller, Vincent, Quasthoff: "An X-linked myopathy with postural muscle atrophy and generalized hypertrophy, termed XMPMA, is caused by mutations in FHL1." in: American journal of human genetics, Vol. 82, Issue 1, pp. 88-99, (2008) (PubMed).

Quinzii, Vu, Min, Tanji, Barral, Grewal, Kattah, Camaño, Otaegui, Kunimatsu, Blake, Wilhelmsen, Rowland, Hays, Bonilla, Hirano: "X-linked dominant scapuloperoneal myopathy is due to a mutation in the gene encoding four-and-a-half-LIM protein 1." in: American journal of human genetics, Vol. 82, Issue 1, pp. 208-13, (2008) (PubMed).

McGrath, Mitchell, Coghill, Robinson, Brown: "Skeletal muscle LIM protein 1 (SLIM1/FHL1) induces alpha 5 beta 1-integrin-dependent myocyte elongation." in: American journal of physiology. Cell physiology, Vol. 285, Issue 6, pp. C1513-26, (2003) (PubMed).

Details zu FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Andere Bezeichnung: FHL1

Hintergrund: LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may play an important role during the early stages of skeletal muscle differentiation, specifically in alpha5beta1-integrin-mediated signaling pathways.
Alias Symbols: KYOT, SLIM, FHL-1, FHL1A, FHL1B, FLH1A, SLIM1, XMPMA, SLIM-1, SLIMMER
Protein Interaction Partner: SUMO2, UBC, SRPK1, SMAD4, SMAD3, SMAD2, LMNA, CSNK1D, NRIP1, ESR1, HIVEP3, SMURF1, UBE2E2, STAT4, RING1, RBPJ, FHL1, KCNA5, PRNP, USP15, HHV8GK18_gp81, SP1, TXNIP, DEAF1, PDE4DIP, AKAP12, EED, HES1, DBN1, CBX4, FHL2, SRF, MYBPC1,
Protein Size: 280

Molekulargewicht: 32 kDa

Gen-ID: 2273

NCBI Accession: NM_001449, NP_001440

UniProt: Q6IB30